The RR interval series is non-stationary and unevenly spaced in time. For estimating its power spectral density (PSD) using traditional techniques like FFT, require resampling at uniform intervals. The researchers have used different interpolation techniques as resampling methods. All these resampling methods introduce the low pass filtering effect in the power spectrum. The lomb transform is a means of obtaining PSD estimates directly from irregularly sampled RR interval series, thus avoiding resampling. In this work, the superiority of Lomb transform method has been established over FFT based approach, after applying linear and cubicspline interpolation as resampling methods, in terms of reproduction of exact frequency locations as well as the relative magnitudes of each spectral component. Keywords—HRV, Lomb Transform, Resampling, RR-intervals.